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polya selection  (New England Biolabs)


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    Structured Review

    New England Biolabs polya selection
    Polya Selection, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6816 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polya+selection/NEBNext+Poly(A)+mRNA+Magnetic+Isolation+Module/pmc13652242-139-6-11
    Average 99 stars, based on 6816 article reviews
    polya selection - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    RNA Sequencing:

    Article Title: Lifelong dietary protein restriction accelerates skeletal muscle loss and reduces muscle fibre size by impairing proteostasis and mitochondrial homeostasis.
    Article Snippet: Total RNA from frozen GAS muscle was extracted and purified by RNeasy mini kits with on-column DNase treatment (Qiagen, Manchester, UK) following manufacturer’s instructions. .. Preparation of dual-indexed, strand-specific RNA-seq library from submitted total RNA was performed using the NEBNext polyA selection and Ultra II Directional RNA library preparation kits (New England Biolabs, UK) as described previously [27]. .. Total RNA integrity was confirmed by an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) with RNA Integrity Number (RIN) > 7.

    Article Title: Lifelong dietary protein restriction accelerates skeletal muscle loss and reduces muscle fibre size by impairing proteostasis and mitochondrial homeostasis
    Article Snippet: Total RNA from frozen GAS muscle was extracted and purified by RNeasy mini kits with on-column DNase treatment (Qiagen, Manchester, UK) following manufacturer's instructions. .. Preparation of dual-indexed, strand-specific RNA-seq library from submitted total RNA was performed using the NEBNext polyA selection and Ultra II Directional RNA library preparation kits (New England Biolabs, UK) as described previously [ ]. .. Total RNA integrity was confirmed by an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) with RNA Integrity Number (RIN) > 7.

    Selection:

    Article Title: Lifelong dietary protein restriction accelerates skeletal muscle loss and reduces muscle fibre size by impairing proteostasis and mitochondrial homeostasis.
    Article Snippet: Total RNA from frozen GAS muscle was extracted and purified by RNeasy mini kits with on-column DNase treatment (Qiagen, Manchester, UK) following manufacturer’s instructions. .. Preparation of dual-indexed, strand-specific RNA-seq library from submitted total RNA was performed using the NEBNext polyA selection and Ultra II Directional RNA library preparation kits (New England Biolabs, UK) as described previously [27]. .. Total RNA integrity was confirmed by an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) with RNA Integrity Number (RIN) > 7.

    Article Title: Translatome analysis reveals microglia and astrocytes to be distinct regulators of inflammation in the hyperacute and acute phases after stroke
    Article Snippet: Sequencing was performed on the Illumina HiSeq 4000 platform to obtain 150 bp paired-end reads. .. The astrocyte samples were sent to Azenta (formerly GeneWiz) for library preparation, using the NEBNext Ultra RNA Library Preparation Kit with polyA selection. .. Sequencing was performed on the Illumina HiSeq 4000 platform to obtain 150 bp paired-end reads.

    Article Title: Transcriptome and DNA methylome analyses of the effects of postharvest 5-azacytidine treatment on pigment and ascorbic acid metabolism in Rosa roxburghii fruit
    Article Snippet: Total RNA was extracted from R. roxburghii fruit samples (Control 0 d, Control 40 d, and 5-azaC 40 d; n = 3 biological replicates) using TRIzolTM reagent (Cat. No. 15596026; Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), with quality control (RIN≥8.0, 28S/ 18S>1.5). .. Strand-specific cDNA libraries were prepared via polyA selection (NEBNext Ultra II RNA Library Prep Kit) and sequenced on Illumina NovaSeq 6000 (150 bp paired-end; 20 M reads/sample). ..

    Article Title: Lifelong dietary protein restriction accelerates skeletal muscle loss and reduces muscle fibre size by impairing proteostasis and mitochondrial homeostasis
    Article Snippet: Total RNA from frozen GAS muscle was extracted and purified by RNeasy mini kits with on-column DNase treatment (Qiagen, Manchester, UK) following manufacturer's instructions. .. Preparation of dual-indexed, strand-specific RNA-seq library from submitted total RNA was performed using the NEBNext polyA selection and Ultra II Directional RNA library preparation kits (New England Biolabs, UK) as described previously [ ]. .. Total RNA integrity was confirmed by an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) with RNA Integrity Number (RIN) > 7.

    Article Title: Day–night gene expression reveals circadian gene disco as a candidate for diel-niche evolution in moths
    Article Snippet: .. RNA libraries were prepared using the NEB polyA selection and NEBNext Ultra II directional stranded kit (New England Biolabs, MA, USA) suitable for low input yields. .. Twelve samples were run on one lane of an Illumina NovaSeq using SP chemistry (Paired-end, 2 × 150 bp sequencing).

    Article Title: Transcriptomic analysis of zebrafish ( Danio rerio ) liver exposed to sediments from the Loa River
    Article Snippet: Library preparation for sequencing was performed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs NEB, cat. no E7770S, United States). .. This process included mRNA isolation using polyA selection with magnetic beads (NEB, cat. no E7490S), followed by RNA fragmentation to generate appropriately sized fragments for sequencing. .. The fragmented RNA was reverse transcribed into complementary DNA (cDNA), and Illumina-specific adapters were ligated.

    Article Title: Impact of low and high lipopolysaccharide doses on the early expression of immune related genes and transcription factors in chicken macrophage-like HD11 cells.
    Article Snippet: .. Briefly, PolyA selection was performed via NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, USA). .. Then, the NEBNext Ultra II RNA Library Preparation Kit (New England Biolabs) was used according to manufacturer protocol, and samples were sequenced with an NovaSeq 6000 (Illumina, San Diego, USA) with the 2 × 150 bp configuration and ~30 M pair-ends reads per sample.

    Article Title: Phenotypic plasticity in coral skeletal features: Molecular signatures from DNA methylation and transcriptional interaction networks
    Article Snippet: .. RNA libraries for 2x150bp paired-end sequencing were prepared using the NEBNext Ultra II Nondirectional Library Prep Kit with polyA selection (New England Biolabs, Inc.). .. Samples were run on one plate of the Illumina NovaSeq platform.

    RNA Library Preparation:

    Article Title: Lifelong dietary protein restriction accelerates skeletal muscle loss and reduces muscle fibre size by impairing proteostasis and mitochondrial homeostasis.
    Article Snippet: Total RNA from frozen GAS muscle was extracted and purified by RNeasy mini kits with on-column DNase treatment (Qiagen, Manchester, UK) following manufacturer’s instructions. .. Preparation of dual-indexed, strand-specific RNA-seq library from submitted total RNA was performed using the NEBNext polyA selection and Ultra II Directional RNA library preparation kits (New England Biolabs, UK) as described previously [27]. .. Total RNA integrity was confirmed by an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) with RNA Integrity Number (RIN) > 7.

    Article Title: Translatome analysis reveals microglia and astrocytes to be distinct regulators of inflammation in the hyperacute and acute phases after stroke
    Article Snippet: Sequencing was performed on the Illumina HiSeq 4000 platform to obtain 150 bp paired-end reads. .. The astrocyte samples were sent to Azenta (formerly GeneWiz) for library preparation, using the NEBNext Ultra RNA Library Preparation Kit with polyA selection. .. Sequencing was performed on the Illumina HiSeq 4000 platform to obtain 150 bp paired-end reads.

    Article Title: Lifelong dietary protein restriction accelerates skeletal muscle loss and reduces muscle fibre size by impairing proteostasis and mitochondrial homeostasis
    Article Snippet: Total RNA from frozen GAS muscle was extracted and purified by RNeasy mini kits with on-column DNase treatment (Qiagen, Manchester, UK) following manufacturer's instructions. .. Preparation of dual-indexed, strand-specific RNA-seq library from submitted total RNA was performed using the NEBNext polyA selection and Ultra II Directional RNA library preparation kits (New England Biolabs, UK) as described previously [ ]. .. Total RNA integrity was confirmed by an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) with RNA Integrity Number (RIN) > 7.

    Isolation:

    Article Title: Transcriptomic analysis of zebrafish ( Danio rerio ) liver exposed to sediments from the Loa River
    Article Snippet: Library preparation for sequencing was performed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs NEB, cat. no E7770S, United States). .. This process included mRNA isolation using polyA selection with magnetic beads (NEB, cat. no E7490S), followed by RNA fragmentation to generate appropriately sized fragments for sequencing. .. The fragmented RNA was reverse transcribed into complementary DNA (cDNA), and Illumina-specific adapters were ligated.

    Article Title: Impact of low and high lipopolysaccharide doses on the early expression of immune related genes and transcription factors in chicken macrophage-like HD11 cells.
    Article Snippet: .. Briefly, PolyA selection was performed via NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, USA). .. Then, the NEBNext Ultra II RNA Library Preparation Kit (New England Biolabs) was used according to manufacturer protocol, and samples were sequenced with an NovaSeq 6000 (Illumina, San Diego, USA) with the 2 × 150 bp configuration and ~30 M pair-ends reads per sample.

    Magnetic Beads:

    Article Title: Transcriptomic analysis of zebrafish ( Danio rerio ) liver exposed to sediments from the Loa River
    Article Snippet: Library preparation for sequencing was performed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs NEB, cat. no E7770S, United States). .. This process included mRNA isolation using polyA selection with magnetic beads (NEB, cat. no E7490S), followed by RNA fragmentation to generate appropriately sized fragments for sequencing. .. The fragmented RNA was reverse transcribed into complementary DNA (cDNA), and Illumina-specific adapters were ligated.

    Sequencing:

    Article Title: Transcriptomic analysis of zebrafish ( Danio rerio ) liver exposed to sediments from the Loa River
    Article Snippet: Library preparation for sequencing was performed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs NEB, cat. no E7770S, United States). .. This process included mRNA isolation using polyA selection with magnetic beads (NEB, cat. no E7490S), followed by RNA fragmentation to generate appropriately sized fragments for sequencing. .. The fragmented RNA was reverse transcribed into complementary DNA (cDNA), and Illumina-specific adapters were ligated.

    Article Title: Phenotypic plasticity in coral skeletal features: Molecular signatures from DNA methylation and transcriptional interaction networks
    Article Snippet: .. RNA libraries for 2x150bp paired-end sequencing were prepared using the NEBNext Ultra II Nondirectional Library Prep Kit with polyA selection (New England Biolabs, Inc.). .. Samples were run on one plate of the Illumina NovaSeq platform.



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    (A) Volcano plot analysis of host transcriptome-wide genes differentially expressed in primary B cells from three independent donors infected with WT or TES2m LMP1 for 21 days. Positive fold changes indicate higher transcripts in cells with TES2m LMP1. (B) PFKFB4 glucose metabolism roles. Glucose is metabolized by glycolysis or by the PPP. Glycolysis flux is regulated via Fructose-2,6-BP, an allosteric regulator of PFK1. PFKFB3 phosphorylates Fructose-6-P into Fructose-2,6-BP while PFKFB4 phosphatase activity converts Fructose-2,6-BP to Fructose-6-P to shunt glycolytic flux to PPP. Created in BioRender. Burton, E. (2026) https://BioRender.com/i0gawc8 . (C) KEGG pathway analysis of transcripts significantly upregulated in cells infected by EBV with WT LMP1 at day 21 post infection. (D) KEGG pathway analysis of transcripts significantly upregulated in cells infected by TES2m EBV at day 21 post infection. (E) PFKFB4 protein levels in WT versus TES2m LMP1 EBV infected cells. Cells from two independent donors were infected with EBV with WT LMP1 or TES2m LMP1 for 22 days. Densitometry values of PFKFB4 vs β-Actin load controls are shown. (F) Effects of LCL LMP1 KO on PFKFB4 <t>mRNA.</t> Median PFKFB4 reads from RNAseq analysis of Cas9+ GM12878 cells with control vs LMP1 targeting sgRNA expression for 48 hours. Shown are data from n=3 RNAseq datasets. (G) PFKFB4 protein levels in LMP1 KO LCLs. Cas9+ GM12878 were mock induced or induced to express LMP1 sgRNA for 48 hours. WCL was extracted and immunoblot was carried out using the indicated antibodies. (H) Effects of TES1 vs TES2 signaling inhibition on LCL PFKFB4 expression. Median PFKFB4 RNAseq reads from RNAseq analysis of Cas9+ GM12878 expressing LMP1 sgRNA together with dox-induced LMP1 TES1m vs WT LMP1 cDNA expression. Shown are data from n=3 RNAseq datasets. (I) Relative PFKFB4 protein abundances from proteomic analysis of primary human B-cells at the indicated days post-infection by the EBV B95.8 strain. Shown are mean ± SD values from n=4 replicates. (J) Effects of exogenous PFKFB4 expression on proliferation of primary B cells infected by EBV with WT versus TES2m LMP1. Peripheral blood B cells from three independent donors were infected with EBV with WT vs TES2m LMP1. After 14 days, cells were transduced with lentivirus control or with lentivirus driving stable PFKFB4 expression. Five days post-selection, cells were re-seeded. Twelve days later, live cell counts were defined. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005. blots are representative of n=3 independent replicates.
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    (A) Volcano plot analysis of host transcriptome-wide genes differentially expressed in primary B cells from three independent donors infected with WT or TES2m LMP1 for 21 days. Positive fold changes indicate higher transcripts in cells with TES2m LMP1. (B) PFKFB4 glucose metabolism roles. Glucose is metabolized by glycolysis or by the PPP. Glycolysis flux is regulated via Fructose-2,6-BP, an allosteric regulator of PFK1. PFKFB3 phosphorylates Fructose-6-P into Fructose-2,6-BP while PFKFB4 phosphatase activity converts Fructose-2,6-BP to Fructose-6-P to shunt glycolytic flux to PPP. Created in BioRender. Burton, E. (2026) https://BioRender.com/i0gawc8 . (C) KEGG pathway analysis of transcripts significantly upregulated in cells infected by EBV with WT LMP1 at day 21 post infection. (D) KEGG pathway analysis of transcripts significantly upregulated in cells infected by TES2m EBV at day 21 post infection. (E) PFKFB4 protein levels in WT versus TES2m LMP1 EBV infected cells. Cells from two independent donors were infected with EBV with WT LMP1 or TES2m LMP1 for 22 days. Densitometry values of PFKFB4 vs β-Actin load controls are shown. (F) Effects of LCL LMP1 KO on PFKFB4 <t>mRNA.</t> Median PFKFB4 reads from RNAseq analysis of Cas9+ GM12878 cells with control vs LMP1 targeting sgRNA expression for 48 hours. Shown are data from n=3 RNAseq datasets. (G) PFKFB4 protein levels in LMP1 KO LCLs. Cas9+ GM12878 were mock induced or induced to express LMP1 sgRNA for 48 hours. WCL was extracted and immunoblot was carried out using the indicated antibodies. (H) Effects of TES1 vs TES2 signaling inhibition on LCL PFKFB4 expression. Median PFKFB4 RNAseq reads from RNAseq analysis of Cas9+ GM12878 expressing LMP1 sgRNA together with dox-induced LMP1 TES1m vs WT LMP1 cDNA expression. Shown are data from n=3 RNAseq datasets. (I) Relative PFKFB4 protein abundances from proteomic analysis of primary human B-cells at the indicated days post-infection by the EBV B95.8 strain. Shown are mean ± SD values from n=4 replicates. (J) Effects of exogenous PFKFB4 expression on proliferation of primary B cells infected by EBV with WT versus TES2m LMP1. Peripheral blood B cells from three independent donors were infected with EBV with WT vs TES2m LMP1. After 14 days, cells were transduced with lentivirus control or with lentivirus driving stable PFKFB4 expression. Five days post-selection, cells were re-seeded. Twelve days later, live cell counts were defined. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005. blots are representative of n=3 independent replicates.
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    Image Search Results


    (A) Volcano plot analysis of host transcriptome-wide genes differentially expressed in primary B cells from three independent donors infected with WT or TES2m LMP1 for 21 days. Positive fold changes indicate higher transcripts in cells with TES2m LMP1. (B) PFKFB4 glucose metabolism roles. Glucose is metabolized by glycolysis or by the PPP. Glycolysis flux is regulated via Fructose-2,6-BP, an allosteric regulator of PFK1. PFKFB3 phosphorylates Fructose-6-P into Fructose-2,6-BP while PFKFB4 phosphatase activity converts Fructose-2,6-BP to Fructose-6-P to shunt glycolytic flux to PPP. Created in BioRender. Burton, E. (2026) https://BioRender.com/i0gawc8 . (C) KEGG pathway analysis of transcripts significantly upregulated in cells infected by EBV with WT LMP1 at day 21 post infection. (D) KEGG pathway analysis of transcripts significantly upregulated in cells infected by TES2m EBV at day 21 post infection. (E) PFKFB4 protein levels in WT versus TES2m LMP1 EBV infected cells. Cells from two independent donors were infected with EBV with WT LMP1 or TES2m LMP1 for 22 days. Densitometry values of PFKFB4 vs β-Actin load controls are shown. (F) Effects of LCL LMP1 KO on PFKFB4 mRNA. Median PFKFB4 reads from RNAseq analysis of Cas9+ GM12878 cells with control vs LMP1 targeting sgRNA expression for 48 hours. Shown are data from n=3 RNAseq datasets. (G) PFKFB4 protein levels in LMP1 KO LCLs. Cas9+ GM12878 were mock induced or induced to express LMP1 sgRNA for 48 hours. WCL was extracted and immunoblot was carried out using the indicated antibodies. (H) Effects of TES1 vs TES2 signaling inhibition on LCL PFKFB4 expression. Median PFKFB4 RNAseq reads from RNAseq analysis of Cas9+ GM12878 expressing LMP1 sgRNA together with dox-induced LMP1 TES1m vs WT LMP1 cDNA expression. Shown are data from n=3 RNAseq datasets. (I) Relative PFKFB4 protein abundances from proteomic analysis of primary human B-cells at the indicated days post-infection by the EBV B95.8 strain. Shown are mean ± SD values from n=4 replicates. (J) Effects of exogenous PFKFB4 expression on proliferation of primary B cells infected by EBV with WT versus TES2m LMP1. Peripheral blood B cells from three independent donors were infected with EBV with WT vs TES2m LMP1. After 14 days, cells were transduced with lentivirus control or with lentivirus driving stable PFKFB4 expression. Five days post-selection, cells were re-seeded. Twelve days later, live cell counts were defined. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005. blots are representative of n=3 independent replicates.

    Journal: bioRxiv

    Article Title: Epstein-Barr Virus Latent Membrane Protein 1 Suppresses Ferroptosis via Pentose Phosphate Pathway and Glutathione Metabolism

    doi: 10.64898/2026.03.09.710628

    Figure Lengend Snippet: (A) Volcano plot analysis of host transcriptome-wide genes differentially expressed in primary B cells from three independent donors infected with WT or TES2m LMP1 for 21 days. Positive fold changes indicate higher transcripts in cells with TES2m LMP1. (B) PFKFB4 glucose metabolism roles. Glucose is metabolized by glycolysis or by the PPP. Glycolysis flux is regulated via Fructose-2,6-BP, an allosteric regulator of PFK1. PFKFB3 phosphorylates Fructose-6-P into Fructose-2,6-BP while PFKFB4 phosphatase activity converts Fructose-2,6-BP to Fructose-6-P to shunt glycolytic flux to PPP. Created in BioRender. Burton, E. (2026) https://BioRender.com/i0gawc8 . (C) KEGG pathway analysis of transcripts significantly upregulated in cells infected by EBV with WT LMP1 at day 21 post infection. (D) KEGG pathway analysis of transcripts significantly upregulated in cells infected by TES2m EBV at day 21 post infection. (E) PFKFB4 protein levels in WT versus TES2m LMP1 EBV infected cells. Cells from two independent donors were infected with EBV with WT LMP1 or TES2m LMP1 for 22 days. Densitometry values of PFKFB4 vs β-Actin load controls are shown. (F) Effects of LCL LMP1 KO on PFKFB4 mRNA. Median PFKFB4 reads from RNAseq analysis of Cas9+ GM12878 cells with control vs LMP1 targeting sgRNA expression for 48 hours. Shown are data from n=3 RNAseq datasets. (G) PFKFB4 protein levels in LMP1 KO LCLs. Cas9+ GM12878 were mock induced or induced to express LMP1 sgRNA for 48 hours. WCL was extracted and immunoblot was carried out using the indicated antibodies. (H) Effects of TES1 vs TES2 signaling inhibition on LCL PFKFB4 expression. Median PFKFB4 RNAseq reads from RNAseq analysis of Cas9+ GM12878 expressing LMP1 sgRNA together with dox-induced LMP1 TES1m vs WT LMP1 cDNA expression. Shown are data from n=3 RNAseq datasets. (I) Relative PFKFB4 protein abundances from proteomic analysis of primary human B-cells at the indicated days post-infection by the EBV B95.8 strain. Shown are mean ± SD values from n=4 replicates. (J) Effects of exogenous PFKFB4 expression on proliferation of primary B cells infected by EBV with WT versus TES2m LMP1. Peripheral blood B cells from three independent donors were infected with EBV with WT vs TES2m LMP1. After 14 days, cells were transduced with lentivirus control or with lentivirus driving stable PFKFB4 expression. Five days post-selection, cells were re-seeded. Twelve days later, live cell counts were defined. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005. blots are representative of n=3 independent replicates.

    Article Snippet: To construct indexed libraries, 1 μg of total RNA was used for polyA mRNA selection using NEBNext Poly(A) mRNA Magnetic Isolation Module (Cat#E7490S), and library preparation with NEBNext Ultra RNA Library Prep with Sample Purification Beads (Cat#E7765S).

    Techniques: Infection, Activity Assay, RNA sequencing, Control, Expressing, Western Blot, Inhibition, Transduction, Selection