polya selection (New England Biolabs)
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Polya Selection, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6816 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polya+selection/NEBNext+Poly(A)+mRNA+Magnetic+Isolation+Module/pmc13652242-139-6-11
Average 99 stars, based on 6816 article reviews
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RNA Sequencing:Article Title: Lifelong dietary protein restriction accelerates skeletal muscle loss and reduces muscle fibre size by impairing proteostasis and mitochondrial homeostasis. Article Snippet: Total RNA from frozen GAS muscle was extracted and purified by RNeasy mini kits with on-column DNase treatment (Qiagen, Manchester, UK) following manufacturer’s instructions. .. Preparation of dual-indexed, strand-specific RNA-seq library from submitted total RNA was performed using the Article Title: Lifelong dietary protein restriction accelerates skeletal muscle loss and reduces muscle fibre size by impairing proteostasis and mitochondrial homeostasis Article Snippet: Total RNA from frozen GAS muscle was extracted and purified by RNeasy mini kits with on-column DNase treatment (Qiagen, Manchester, UK) following manufacturer's instructions. .. Preparation of dual-indexed, strand-specific RNA-seq library from submitted total RNA was performed using the Selection:Article Title: Lifelong dietary protein restriction accelerates skeletal muscle loss and reduces muscle fibre size by impairing proteostasis and mitochondrial homeostasis. Article Snippet: Total RNA from frozen GAS muscle was extracted and purified by RNeasy mini kits with on-column DNase treatment (Qiagen, Manchester, UK) following manufacturer’s instructions. .. Preparation of dual-indexed, strand-specific RNA-seq library from submitted total RNA was performed using the Article Title: Translatome analysis reveals microglia and astrocytes to be distinct regulators of inflammation in the hyperacute and acute phases after stroke Article Snippet: Sequencing was performed on the Illumina HiSeq 4000 platform to obtain 150 bp paired-end reads. .. The astrocyte samples were sent to Azenta (formerly GeneWiz) for library preparation, using the NEBNext Ultra RNA Library Preparation Kit with Article Title: Transcriptome and DNA methylome analyses of the effects of postharvest 5-azacytidine treatment on pigment and ascorbic acid metabolism in Rosa roxburghii fruit Article Snippet: Total RNA was extracted from R. roxburghii fruit samples (Control 0 d, Control 40 d, and 5-azaC 40 d; n = 3 biological replicates) using TRIzolTM reagent (Cat. No. 15596026; Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), with quality control (RIN≥8.0, 28S/ 18S>1.5). .. Strand-specific cDNA libraries were prepared via Article Title: Lifelong dietary protein restriction accelerates skeletal muscle loss and reduces muscle fibre size by impairing proteostasis and mitochondrial homeostasis Article Snippet: Total RNA from frozen GAS muscle was extracted and purified by RNeasy mini kits with on-column DNase treatment (Qiagen, Manchester, UK) following manufacturer's instructions. .. Preparation of dual-indexed, strand-specific RNA-seq library from submitted total RNA was performed using the Article Title: Day–night gene expression reveals circadian gene disco as a candidate for diel-niche evolution in moths Article Snippet: .. RNA libraries were prepared using the Article Title: Transcriptomic analysis of zebrafish ( Danio rerio ) liver exposed to sediments from the Loa River Article Snippet: Library preparation for sequencing was performed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs NEB, cat. no E7770S, United States). .. This process included mRNA isolation using Article Title: Impact of low and high lipopolysaccharide doses on the early expression of immune related genes and transcription factors in chicken macrophage-like HD11 cells. Article Snippet: .. Briefly, Article Title: Phenotypic plasticity in coral skeletal features: Molecular signatures from DNA methylation and transcriptional interaction networks Article Snippet: .. RNA libraries for 2x150bp paired-end sequencing were prepared using the NEBNext Ultra II Nondirectional Library Prep Kit with RNA Library Preparation:Article Title: Lifelong dietary protein restriction accelerates skeletal muscle loss and reduces muscle fibre size by impairing proteostasis and mitochondrial homeostasis. Article Snippet: Total RNA from frozen GAS muscle was extracted and purified by RNeasy mini kits with on-column DNase treatment (Qiagen, Manchester, UK) following manufacturer’s instructions. .. Preparation of dual-indexed, strand-specific RNA-seq library from submitted total RNA was performed using the Article Title: Translatome analysis reveals microglia and astrocytes to be distinct regulators of inflammation in the hyperacute and acute phases after stroke Article Snippet: Sequencing was performed on the Illumina HiSeq 4000 platform to obtain 150 bp paired-end reads. .. The astrocyte samples were sent to Azenta (formerly GeneWiz) for library preparation, using the NEBNext Ultra RNA Library Preparation Kit with Article Title: Lifelong dietary protein restriction accelerates skeletal muscle loss and reduces muscle fibre size by impairing proteostasis and mitochondrial homeostasis Article Snippet: Total RNA from frozen GAS muscle was extracted and purified by RNeasy mini kits with on-column DNase treatment (Qiagen, Manchester, UK) following manufacturer's instructions. .. Preparation of dual-indexed, strand-specific RNA-seq library from submitted total RNA was performed using the Isolation:Article Title: Transcriptomic analysis of zebrafish ( Danio rerio ) liver exposed to sediments from the Loa River Article Snippet: Library preparation for sequencing was performed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs NEB, cat. no E7770S, United States). .. This process included mRNA isolation using Article Title: Impact of low and high lipopolysaccharide doses on the early expression of immune related genes and transcription factors in chicken macrophage-like HD11 cells. Article Snippet: .. Briefly, Magnetic Beads:Article Title: Transcriptomic analysis of zebrafish ( Danio rerio ) liver exposed to sediments from the Loa River Article Snippet: Library preparation for sequencing was performed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs NEB, cat. no E7770S, United States). .. This process included mRNA isolation using Sequencing:Article Title: Transcriptomic analysis of zebrafish ( Danio rerio ) liver exposed to sediments from the Loa River Article Snippet: Library preparation for sequencing was performed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs NEB, cat. no E7770S, United States). .. This process included mRNA isolation using Article Title: Phenotypic plasticity in coral skeletal features: Molecular signatures from DNA methylation and transcriptional interaction networks Article Snippet: .. RNA libraries for 2x150bp paired-end sequencing were prepared using the NEBNext Ultra II Nondirectional Library Prep Kit with |
